Biotech Perishables
biotech perishables updated may 2016
Biotech Perishables Updated May 2016
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| Perishables | Short term Storage | Long Term Storage | Shelf Life (Short Term Storage) | Shelf Life (Long Term Storage) | Shipping Method |
| Cell Lysis Solution | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| 70% Ethanol | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Cell Lysis Solution | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| 70% Ethanol | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Lysis Buffer (Detergent) | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| NaCl | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Ethanol | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Cell Lysis Solution and Filters | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| DNA Precipitation Solution | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Tris-EDTA (TE) Solution | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Potassium Acetate Solution | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Glucose-Tris-EDTA (GTE) Solution | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Sodium Hydroxide Solution (4 N) | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Sodium Dodecyl Sulfate (10%) | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Isopropanol | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Ethanol (95%) | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Cell Lysis Solution | Refrigerator (~4°C) | Refrigerator (~4°C) | 12 months | 12 months | |
| Protein Precipitation Solution | Refrigerator (~4°C) | Refrigerator (~4°C) | 12 months | 12 months | |
| DNA Hydration Solution | Refrigerator (~4°C) | Refrigerator (~4°C) | 12 months | 12 months | |
| RNaseA | Freezer (~-20°C) | Freezer (~-20°C) | 12 months | 12 months | |
| Lambda Genomic DNA Standard | Refrigerator (~4°C) | Freezer (~-20°C) | 4 weeks | 12 months | |
| CarolinaBLU™ DNA Stain Concentrate | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Distilled Water | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| DNA Quantitation Plates | Room Temp. (~21°C) | Room Temp. (~21°C) | 4 weeks | 4 weeks | |
| Euglena Culture | Room Temp. (~21°C) | Room Temp. (~21°C) | 1 week | 1 week | |
| Elodea Leaves | Room Temp. (~21°C) | Room Temp. (~21°C) | 1 week | 1 week | |
| Packet of Yeast | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Materials for Preparing Slides and Staining Cells | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Protoslo® Quieting Solution | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Drosophila Fruitflies (Mixed phenotypes) | Room Temp. (~21°C) | Room Temp. (~21°C) | Variable | Variable | |
| Prepoured AMP Plates | Refrigerator (~4°C) | Refrigerator (~4°C) | 2 weeks | 4 weeks | |
| E. coli | Room Temp. (~21°C) | Room Temp. (~21°C) | 2 weeks | 4 weeks | |
| Transformation Buffer | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| LB Broth | Refrigerator (~4°C) | Refrigerator (~4°C) | 12 months | 12 months | |
| Plasmid DNA | Refrigerator (~4°C) | Freezer (~-20°C) | 3 months | 24 months | |
| LB Plates | Room Temp. (~21°C) | Room Temp. (~21°C) | 2 weeks | 4 weeks | |
| HIC Resin | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Lysis Buffer | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Binding Buffer | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Wash Buffer | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| elution buffer | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| LB Broth | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Ampicillin | Refrigerator (~4°C) | Freezer (~-20°C) | 2 weeks | 6-12 months | |
| Equilibration Buffer | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Polyacrylamide Gels | Refrigerator (~4°C) | Refrigerator (~4°C) | see packaging/use within 2 weeks | see packaging/use within 2 weeks | |
| 5x Tris-Glycine SDS Buffer | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Protein Marker | Refrigerator (~4°C) | Refrigerator (~4°C) | 3 months | 12 months | |
| COOMASIE Stain | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Sample Loading Buffer | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Plasmid DNA Solution | Refrigerator (~4°C) | Freezer (~-20°C) | 1 month | 6-12 months | |
| Ampicillin | Refrigerator (~4°C) | Freezer (~-20°C) | 2 weeks | 6-12 months | |
| Transformation Buffer and Luria Broth | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Prepoured plates | Refrigerator (~4°C) | Refrigerator (~4°C) | 2 weeks | 4 weeks | |
| Plasmid DNA Solution | Refrigerator (~4°C) | Freezer (~-20°C) | 1 month | 6-12 months | |
| Ampicillin | Refrigerator (~4°C) | Freezer (~-20°C) | 2 weeks | 6-12 months | |
| pBLU® Plasmid | Refrigerator (~4°C) | Freezer (~-20°C) | 2 weeks | 6-12 months | |
| Transformation Buffer and Luria Broth | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Prepared Ampicillin/X-gal Solution | Refrigerator (~4°C) | Freezer (~-20°C) | 2 weeks | 6-12 months | |
| E. coli | Refrigerator (~4°C) | Refrigerator (~4°C) | 2 weeks | 4 weeks | |
| Plasmid DNA Solution | Room Temp. (~21°C) | Freezer (~-20°C) | 1 month | 6-12 months | |
| Transformation Buffer and Luria Broth | Refrigerator (~4°C) | Freezer (~-20°C) | 1 month | 12 months | |
| Prepoured Plates | Refrigerator (~4°C) | Refrigerator (~4°C) | 2 weeks | 2 weeks | |
| slant culture | Refrigerator (~4°C) | Refrigerator (~4°C) | 2 weeks | 1 month | |
| streaked plate | Refrigerator (~4°C) | Refrigerator (~4°C) | 2 weeks | 1 month | |
| Refrigerator (~4°C) | Refrigerator (~4°C) | 2 weeks | 1 month | ||
| Refrigerator (~4°C) | Refrigerator (~4°C) | 2 weeks | 1 month | ||
| Freezer (~-70°C) | Freezer (~-70°C) | 2 weeks | 1 month | ||
| Plasmid DNA Fragments | Refrigerator (~4°C) | Freezer (~-20°C) | 1 month | 12 months | |
| E. coli | Refrigerator (~4°C) | Refrigerator (~4°C) | 2 weeks | 4 weeks | |
| Ligation Solution | Freezer (~-20°C) | Freezer (~-20°C) | 12 months | 12 months | |
| Kanamycin Plates | Refrigerator (~4°C) | Refrigerator (~4°C) | 2 weeks | 2 weeks | |
| Ampicillin Plates | Refrigerator (~4°C) | Refrigerator (~4°C) | 2 weeks | 2 weeks | |
| Transformation Buffer and LB Broth | Room Temp. (~21°C) | Room Temp. (~21°C) | 1 month | 12 months | |
| Kanamycin | Refrigerator (~4°C) | Freezer (~-20°C) | 1 month | 12 months | |
| Ampicillin | Refrigerator (~4°C) | Freezer (~-20°C) | 2 weeks | 12 months | |
| Ready-to-pour Luria broth agar plates | Room Temp. (~21°C) | Refrigerator (~4°C) | 6 months | 12 months | |
| Luria broth | Room Temp. (~21°C) | Room Temp. (~21°C) | 6 months | 12 months | |
| E. coli Strains | Refrigerator (~4°C) | Refrigerator (~4°C) | 3 months | 3 months | |
| Bacteriophage Lysate | Freezer (~-20°C) | Freezer (~-20°C) | 12 months | 12 months | |
| Ampicillin | Refrigerator (~4°C) | Freezer (~-20°C) | 2 weeks | 6-12 months | |
| Luria Broth | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Ready-to-Pour Sterile Agar | Room Temp. (~21°C) | Refrigerator (~4°C) | 6 months | 12 months | |
| Strains of E.coli | Refrigerator (~4°C) | Refrigerator (~4°C) | 2 weeks | 4 weeks | |
| Antibiotic Solutions | Refrigerator (~4°C) | Freezer (~-20°C) | 2 weeks | 6-12 months | |
| Ready-to-Pour Sterile Agar | Room Temp. (~21°C) | Refrigerator (~4°C) | 6 months | 12 months | |
| Luria Broth (for overnight cultures) | Room Temp. (~21°C) | Room Temp. (~21°C) | 6 months | 12 months | |
| E. coli Strain I (Str) | Refrigerator (~4°C) | Refrigerator (~4°C) | 2 weeks | 4 weeks | |
| E. Coli Strain II (Amp and Nal) | Refrigerator (~4°C) | Refrigerator (~4°C) | 2 weeks | 4 weeks | |
| Naladixic Acid Solution, 0.5%, 3.5 mL | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Streptomycin Solution, 2%, 3.5 mL | Refrigerator (~4°C) | Freezer (~-20°C) | 2 weeks | 3 months | |
| Ampicillin Solution, 1%, 3.5 mL | Refrigerator (~4°C) | Freezer (~-20°C) | 2 weeks | 3 months | |
| LB Broth | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| LB Agar | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Agarose Powder | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| TBE Buffer | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Dye Samples | Room Temp. (~21°C) | Freezer (~-20°C) | 4 weeks | 12 months | |
| Agarose | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| 20x TBE | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| CarolinaBLU™ Stain ( | Room Temp. (~21°C) | Freezer (~-20°C) | 6 weeks | 12 months | |
| Suspect 1 DNA | Room Temp. (~21°C) | Freezer (~-20°C) | 6 weeks | 12 months | |
| Suspect 2 DNA | Room Temp. (~21°C) | Freezer (~-20°C) | 6 weeks | 12 months | |
| Victim DNA | Room Temp. (~21°C) | Freezer (~-20°C) | 6 weeks | 12 months | |
| Evidence DNA | Room Temp. (~21°C) | Freezer (~-20°C) | 6 weeks | 12 months | |
| Agarose Powder and TBE Buffer | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| CarolinaBLU™ DNA Stain | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Predigested DNA Samples | Freezer (~-20°C) | Freezer (~-20°C) | 12 months | 12 months | |
| DNA Samples | Freezer (~-20°C) | Freezer (~-20°C) | 12 months | 12 months | |
| DNA Markers | Freezer (~-20°C) | Freezer (~-20°C) | 12 months | 12 months | |
| Restriction Enzymes | Freezer (~-20°C) | Freezer (~-20°C) | 12 months | 12 months | |
| Agarose and TBE Buffer | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| CarolinaBLU™ DNA Stain | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| DNA Samples | Freezer (~-20°C) | Freezer (~-20°C) | 12 months | 12 months | |
| DNA Markers | Freezer (~-20°C) | Freezer (~-20°C) | 12 months | 12 months | |
| Restriction Enzymes | Freezer (~-20°C) | Freezer (~-20°C) | 12 months | 12 months | |
| Agarose and TBE Buffer | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| CarolinaBLU™ DNA Stain | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Agarose Powder and TBE Buffer | Room Temp. (~21°C) | Room Temp. (~21°C) | 6-12 months | 6-12 months | |
| CarolinaBLU™ DNA Stain | Room Temp. (~21°C) | Room Temp. (~21°C) | 6-12 months | 6-12 months | |
| Lambda DNA and Restriction Enzymes | Room Temp. (~21°C) | Room Temp. (~21°C) | 6-12 months | 6-12 months | |
| Agarose Powder | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| TBE Buffer | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Bromophenol Blue | Room Temp. (~21°C) | Freezer (~-20°C) | 12 months | 12 months | |
| Methyl Orange | Room Temp. (~21°C) | Freezer (~-20°C) | 12 months | 12 months | |
| Ponceau | Room Temp. (~21°C) | Freezer (~-20°C) | 12 months | 12 months | |
| Xylene Cyano | Room Temp. (~21°C) | Freezer (~-20°C) | 12 months | 12 months | |
| Pyronin Y | Room Temp. (~21°C) | Freezer (~-20°C) | 12 months | 12 months | |
| Unknowns (1-3) | Room Temp. (~21°C) | Freezer (~-20°C) | 12 months | 12 months | |
| Lambda DNA and Restriction Enzymes | Freezer (~-20°C) | Freezer (~-20°C) | 12 months | 12 months | |
| Restriction Buffer | Freezer (~-20°C) | Freezer (~-20°C) | 12 months | 12 months | |
| TBE Buffer | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Loading Dye | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| ethidium bromide | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Agarose | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Lambda DNA and Restriction Enzymes | Freezer (~-20°C) | Freezer (~-20°C) | 12 months | 12 months | |
| Restriction Buffer | Freezer (~-20°C) | Freezer (~-20°C) | 12 months | 12 months | |
| TBE Buffer | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Loading Dye | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| CarolinaBLU | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Agarose | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| DNA and Restriction Enzymes | Room Temp. (~21°C) | Room Temp. (~21°C) | 6 weeks | 12 months | |
| DNA and Restriction Enzymes | Room Temp. (~21°C) | Room Temp. (~21°C) | 6 weeks | 12 months | |
| DNA and Restriction Enzymes | Room Temp. (~21°C) | Room Temp. (~21°C) | 6 weeks | 12 months | |
| ethidium bromide | Room Temp. (~21°C) | Room Temp. (~21°C) | 6 weeks | 12 months | |
| Lambda DNA | Room Temp. (~21°C) | Freezer (~-20°C) | 6 weeks | 12 months | |
| Lambda DNA cut with EcoR1 | Room Temp. (~21°C) | Freezer (~-20°C) | 6 weeks | 12 months | |
| Lambda DNA cut with HindIII | Room Temp. (~21°C) | Freezer (~-20°C) | 6 weeks | 12 months | |
| CarolinaBLU™ Final Stain | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| CarolinaBLU™ Gel and Buffer Stain | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| TAE Buffer | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Agarose | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Sets of DNA Sample | Room Temp. (~21°C) | Freezer (~-20°C) | 6 weeks | 12 months | |
| Agarose | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| 20x TBE Buffer | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| CarolinaBLU™ Final Stain | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| CarolinaBLU™ Gel and Buffer Stain | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Sets of DNA Sample | Room Temp. (~21°C) | Freezer (~-20°C) | 6 weeks | 12 months | |
| Agarose | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| TBE Buffer | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| CarolinaBLU™ DNA Stain | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| DNA Samples A-F | Refrigerator (~4°C) | Freezer (~-20°C) | 6 weeks | 12 months | |
| Agarose | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| 20x LB® Electrophoresis Medium | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| E. coli slants | Refrigerator (~4°C) | Refrigerator (~4°C) | 2 weeks | 4 weeks | |
| Restriction Buffer | Freezer (~-20°C) | Freezer (~-20°C) | 6-12 months | 6-12 months | |
| Restriction Enzymes | Freezer (~-20°C) | Freezer (~-20°C) | 6-12 months | 6-12 months | |
| RNase | Freezer (~-20°C) | Freezer (~-20°C) | 6-12 months | 6-12 months | |
| "Evidence DNA" and "Suspect DNA" | Freezer (~-20°C) | Freezer (~-20°C) | 6-12 months | 6-12 months | |
| Luria Broth | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Glucose-Tris-EDTA | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Potassium Acetate-Acetic Acid and Isopropanol | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Ethanol (95%) | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Tris-EDTA Buffer | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Sodium Dodecyl Sulfate | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Sodium Hydroxide | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Loading Dye | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| TBE Electrophoresis Buffer | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Agarose | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Ethidium Bromide | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| CarolinaBLU™ Final Stain | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| CarolinaBLU™ Gel and Buffer Stain | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Different Restriction Digests of Plasmid DNA | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Molecular Weight Standard | Room Temp. (~21°C) | Freezer (~-20°C) | 1 month | 12 months | |
| Agarose | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| TBE Buffer | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| CarolinaBLU™ Stain | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Lambda DNA | Refrigerator (~4°C) | Freezer (~-20°C) | 1 month | 12 months | |
| Restriction Enzymes | Freezer (~-20°C) | Freezer (~-20°C) | 1 month | 12 months | |
| Restriction Buffer | Freezer (~-20°C) | Freezer (~-20°C) | 1 month | 12 months | |
| 2x Denaturization Buffer | Room Temp. (~21°C) | Room Temp. (~21°C) | 6-12 months | 6-12 months | |
| 2x Neutralization Buffer | Room Temp. (~21°C) | Room Temp. (~21°C) | 6-12 months | 6-12 months | |
| 20x SSC w/SDS | Room Temp. (~21°C) | Room Temp. (~21°C) | 6-12 months | 6-12 months | |
| Prehybridization/Hybridization Buffer | Room Temp. (~21°C) | Room Temp. (~21°C) | 6-12 months | 6-12 months | |
| Blocking Agent | Room Temp. (~21°C) | Room Temp. (~21°C) | 6-12 months | 6-12 months | |
| Buffer for Detection Steps | Room Temp. (~21°C) | Room Temp. (~21°C) | 6-12 months | 6-12 months | |
| NBT/BCIP Color Development Solution | Refrigerator (~4°C) | Refrigerator (~4°C) | 6-12 months | 6-12 months | |
| Biotinylated Probe | Refrigerator (~4°C) | Freezer (~-20°C) | 1 month | 12 months | |
| Streptavidin-Alkaline Phosphatase Conjugate | Refrigerator (~4°C) | Do Not Freeze! | 6-12 months | 6-12 months | |
| Precut DNA | Refrigerator (~4°C) | Freezer (~-20°C) | 1 month | 12 months | |
| Agarose | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| TBE Buffer | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Plant rcbL Primer/Loading Dye Mix | Freezer (~-20°C) | Freezer (~-20°C) | 1 month | 12 months | |
| Fish COI Primer/Loading Dye Mix | Freezer (~-20°C) | Freezer (~-20°C) | 1 month | 12 months | |
| Insect-mammal Primer/Loading Dye Mix | Freezer (~-20°C) | Freezer (~-20°C) | 1 month | 12 months | |
| RNAse Solution | Freezer (~-20°C) | Freezer (~-20°C) | 1 month | 12 months | |
| pBR322/BstNI Markers | Freezer (~-20°C) | Freezer (~-20°C) | 1 month | 12 months | |
| Nuclei Lysis Solution | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Protein Precipitation Solution | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Isopropanol | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Ethanol | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| DNA Rehydration Buffer | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Mineral Oil | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Ready to go PCR beads | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Agarose | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| TBE Buffer | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| CarolinaBLU™ Final Stain | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| CarolinaBLU™ Gel and Buffer Stain | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| GelGreen DNA Stain | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Plant rcbL Primer/Loading Dye Mix | Freezer (~-20°C) | Freezer (~-20°C) | 12 months | 12 months | |
| Fish COI Primer/Loading Dye Mix | Freezer (~-20°C) | Freezer (~-20°C) | 12 months | 12 months | |
| Insect-mammal Primer/Loading Dye Mix | Freezer (~-20°C) | Freezer (~-20°C) | 12 months | 12 months | |
| RNAse Solution | Freezer (~-20°C) | Freezer (~-20°C) | 12 months | 12 months | |
| pBR322/BstNI Markers | Freezer (~-20°C) | Freezer (~-20°C) | 12 months | 12 months | |
| Nuclei Lysis Solution | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Protein Precipitation Solution | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Isopropanol | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Ethanol | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| DNA Rehydration Buffer | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Mineral Oil | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Ready to go PCR beads | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Agarose | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| TBE Buffer | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Halobacterium sp. KBT-1 (mutant) | 37°C-42°C | 37°C-42°C | 3-4 Weeks (before subculturing) | 3-4 Weeks (before subculturing) | |
| Halobacterium sp. NRC-1 (wild type) | 37°C-42°C | 37°C-42°C | 3-4 Weeks (before subculturing) | 3-4 Weeks (before subculturing) | |
| Primer-Loading Dye Mix | Freezer (~-20°C) | Freezer (~-20°C) | 12 months | 12 months | |
| 1-kb Ladder/DNA Marker | Freezer (~-20°C) | Freezer (~-20°C) | 12 months | 12 months | |
| Ready-To-Go-Beads™ | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| CarolinaBLU™ Final Stain | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| CarolinaBLU™ Gel and Buffer Stain | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| GelGreen DNA Stain | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Chelex® Resin | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Proteinase K, | Freezer (~-20°C) | Freezer (~-20°C) | 12 months | 12 months | |
| PV92B Primer/Loading Dye Mix | Freezer (~-20°C) | Freezer (~-20°C) | 12 months | 12 months | |
| Ready-To-Go-Beads™ | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| pBr322/B s t NI Markers, 0.075 μg/μL | Freezer (~-20°C) | Freezer (~-20°C) | 12 months | 12 months | |
| CarolinaBLU™ Final Stain, | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| CarolinaBLU™ Gel and Buffer Stain | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Ethidium Bromide | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Chelex® Resin | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Proteinase K, | Freezer (~-20°C) | Freezer (~-20°C) | 12 months | 12 months | |
| mtDNA Primer/Loading Dye | Freezer (~-20°C) | Freezer (~-20°C) | 12 months | 12 months | |
| pBr322/B s t NI Markers, 0.075 μg/μL | Freezer (~-20°C) | Freezer (~-20°C) | 12 months | 12 months | |
| Ready-To-Go-Beads™ | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| CarolinaBLU™ Final Stain, | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| CarolinaBLU™ Gel and Buffer Stain | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Ethidium Bromide | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Proteinase K, | Freezer (~-20°C) | Freezer (~-20°C) | 12 months | 12 months | |
| Primer Mix | Freezer (~-20°C) | Freezer (~-20°C) | 12 months | 12 months | |
| DNA Marker | Freezer (~-20°C) | Freezer (~-20°C) | 12 months | 12 months | |
| Restriction Enzyme with Buffer and BSA | Freezer (~-20°C) | Freezer (~-20°C) | 12 months | 12 months | |
| Chelex | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Ready-to-Go Beads™ | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Loading Dye | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| pBr322/B s t NI Markers, 0.075 μg/μL | Freezer (~-20°C) | Freezer (~-20°C) | 12 months | 12 months | |
| HaeIII Restriction Enzyme | Freezer (~-20°C) | Freezer (~-20°C) | 12 months | 12 months | |
| PTC Primer/Loading Dye Mix | Freezer (~-20°C) | Freezer (~-20°C) | 12 months | 12 months | |
| Chelex® Resin | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Ready-to-Go&trade PCR Beads | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| CarolinaBLU™ Final Stain, | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| CarolinaBLU™ Gel and Buffer Stain | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Ethidium Bromide | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Chelex | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| proteinase K | Freezer (~-20°C) | Freezer (~-20°C) | 12 months | 12 months | |
| Ready-to-go Beads™ | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Primer/Loading Dye Mix | Freezer (~-20°C) | Freezer (~-20°C) | 12 months | 12 months | |
| DNA Markers | Freezer (~-20°C) | Freezer (~-20°C) | 12 months | 12 months | |
| CarolinaBLU™ Final Stain, | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| CarolinaBLU™ Gel and Buffer Stain | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Ethidium Bromide | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Wild-Type Soybean Seeds | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Roundup Ready® Soybean Tissue | Freezer (~-20°C) | Freezer (~-20°C) | 12 months | 12 months | |
| pBR322/BstN1 Markers | Freezer (~-20°C) | Freezer (~-20°C) | 12 months | 12 months | |
| Tris/EDTA (TE) Buffer with RNase A | Freezer (~-20°C) | Freezer (~-20°C) | 12 months | 12 months | |
| 35S Primer/Loading Dye Mix | Freezer (~-20°C) | Freezer (~-20°C) | 12 months | 12 months | |
| Tubulin Primer/Loading Dye Mix | Freezer (~-20°C) | Freezer (~-20°C) | 12 months | 12 months | |
| Edwards' Buffer | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Isopropanol, 100% | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Mineral Oil | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Ready-To-Go-Beads™ | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| CarolinaBLU™ Final Stain, | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| CarolinaBLU™ Gel and Buffer Stain | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Ethidium Bromide | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Lambda DNA | Freezer (~-20°C) | Freezer (~-20°C) | 12 months | 12 months | |
| Primer Mix | Freezer (~-20°C) | Freezer (~-20°C) | 12 months | 12 months | |
| Loading Dye | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Lambda/HindIII DNA Molecular Weight Standard | Freezer (~-20°C) | Freezer (~-20°C) | 12 months | 12 months | |
| Mineral Oil | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| PCR Tubes Containing Ready-To-Go-Beads | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| CarolinaBLU™ Final Stain, | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| CarolinaBLU™ Gel and Buffer Stain | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Ethidium Bromide | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| DNA Chips, Gene 1-6 | Room Temp. (~21°C) | Room Temp. (~21°C) | 6-12 months | 6-12 months | |
| Hybridization Buffer | Room Temp. (~21°C) | Room Temp. (~21°C) | 6-12 months | 6-12 months | |
| Cation-Exchange Membrane | Room Temp. (~21°C) | Room Temp. (~21°C) | 6-12 months | 6-12 months | |
| Glucose | Room Temp. (~21°C) | Room Temp. (~21°C) | 6-12 months | 6-12 months | |
| Methylene Blue | Room Temp. (~21°C) | Room Temp. (~21°C) | 6-12 months | 6-12 months | |
| Potassium Hexacyanoferrate | Room Temp. (~21°C) | Room Temp. (~21°C) | 6-12 months | 6-12 months | |
| Active Dry Yeast | Room Temp. (~21°C) | Room Temp. (~21°C) | 6-12 months | 6-12 months | |
| TBE Buffer | Room Temp. (~21°C) | Room Temp. (~21°C) | 6-12 months | 6-12 months | |
| Albumin/Hemoglobin/Bromophenol Blue Sample | Refrigerator (~4°C) | Refrigerator (~4°C) | 6-12 months | 6-12 months | |
| Agarose | Room Temp. (~21°C) | Room Temp. (~21°C) | 6-12 months | 6-12 months | |
| Fine-Sieving Agarose | Room Temp. (~21°C) | Room Temp. (~21°C) | 6-12 months | 6-12 months | |
| Fish Protein Sample Set | Freezer (~-20°C) | Freezer (~-20°C) | 6-12 months | 6-12 months | |
| Protein Size Standard | Refrigerator (~4°C) | Refrigerator (~4°C) | 6-12 months | 6-12 months | |
| Tris-Glycine SDS Buffer | Room Temp. (~21°C) | Room Temp. (~21°C) | 6-12 months | 6-12 months | |
| COOMASSIE® Protein Stain Solution | Room Temp. (~21°C) | Room Temp. (~21°C) | 6-12 months | 6-12 months | |
| Destain Solution | Room Temp. (~21°C) | Room Temp. (~21°C) | 6-12 months | 6-12 months | |
| Melt n Pour Agarose | Room Temp. (~21°C) | Room Temp. (~21°C) | 6-12 months | 6-12 months | |
| Enzyme Running Buffer | Room Temp. (~21°C) | Room Temp. (~21°C) | 6-12 months | 6-12 months | |
| Nonreducing Protein Loading Dye | Refrigerator (~4°C) | Refrigerator (~4°C) | 6-12 months | 6-12 months | |
| Pure Alpha Amylase (for positive control) | Refrigerator (~4°C) | Refrigerator (~4°C) | 6-12 months | 6-12 months | |
| COOMASSIE® Stain and Destain Solutions | Room Temp. (~21°C) | Room Temp. (~21°C) | 6-12 months | 6-12 months | |
| Amylase Activation Solution | Room Temp. (~21°C) | Room Temp. (~21°C) | 6-12 months | 6-12 months | |
| Positive Control | Refrigerator (~4°C) | Freezer (~-20°C) | 1 month | 12 months | |
| Negative Control | Refrigerator (~4°C) | Freezer (~-20°C) | 1 month | 12 months | |
| Simulated Patient Samples | Refrigerator (~4°C) | Freezer (~-20°C) | 1 month | 12 months | |
| Simulated Antigen | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Simulated Secondary Antibody | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Simulated Chromagen | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Reagent A | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Reagent B | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| BSA Standard | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Powdered Milk | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Diet Drink | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| E. coli Slant | Refrigerator (~4°C) | Refrigerator (~4°C) | 2 weeks | 4 weeks | |
| Ampicillin-Resistant E. coli Slant | Refrigerator (~4°C) | Refrigerator (~4°C) | 2 weeks | 4 weeks | |
| Ampicillin | Refrigerator (~4°C) | Freezer (~-20°C) | 2 weeks | 6-12 months | |
| LB Plate | Refrigerator (~4°C) | Refrigerator (~4°C) | 2 weeks | 4 weeks | |
| LB Plate and LB/AMP Plate | Refrigerator (~4°C) | Refrigerator (~4°C) | 2 weeks | 4 weeks | |
| LB Broth | Room Temp. (~21°C) | Freezer (~-20°C) | 1 month | 12 months | |
| Penicillin G and Phenol Red | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| 0.8% EtBr | Room Temp. (~21°C) | Room Temp. (~21°C) | 1 month | 6-8 months to a year | |
| 2% EtBr | Room Temp. (~21°C) | Room Temp. (~21°C) | 1 month | 6-8 months to a year | |
| 2% SYBR Safe | Room Temp. (~21°C) | Room Temp. (~21°C) | 2 weeks | 4 weeks | |
| Ampicillin | Refrigerator (~4°C) | Freezer (~-20°C) | 2 weeks | 6-12 months | |
| Kanamycin | Refrigerator (~4°C) | Freezer (~-20°C) | 1 month | 12 months | |
| pGREEN Plasmid | Refrigerator (~4°C) | Freezer (~-20°C) | 2 weeks | 6-12 months | |
| pAMP Plasmid | Refrigerator (~4°C) | Freezer (~-20°C) | 2 weeks | 6-12 months | |
| pKAN Plasmid | Refrigerator (~4°C) | Freezer (~-20°C) | 2 weeks | 6-12 months | |
| MM294 Slant Culture | Refrigerator (~4°C) | Refrigerator (~4°C) | 2 weeks | 2 weeks | |
| Child #1 DNA | Room Temp. (~21°C) | Freezer (~-20°C) | 6 weeks | 12 months | |
| Child #2 DNA | Room Temp. (~21°C) | Freezer (~-20°C) | 6 weeks | 12 months | |
| No DNA Template Control | Room Temp. (~21°C) | Freezer (~-20°C) | 6 weeks | 12 months | |
| Wild Type Control DNA | Room Temp. (~21°C) | Freezer (~-20°C) | 6 weeks | 12 months | |
| Mutated Control DNA | Room Temp. (~21°C) | Freezer (~-20°C) | 6 weeks | 12 months | |
| HindIII/EcoRI Cut Lambda DNA | Room Temp. (~21°C) | Room Temp. (~21°C) | 6 weeks | 12 months | |
| Agarose | Room Temp. (~21°C) | Room Temp. (~21°C) | 6-12 months | 6-12 months | |
| TAE Buffer | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Carolina Blu | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Arabidopsis Seed Pack from Ler (Landsberg erecta) Wild Type | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Arabidopsis Seed Pack from fwa-1 (flowering wageningen) Mutant | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| RNAse Solution | Freezer (~-20°C) | Freezer (~-20°C) | 12 months | 12 months | |
| FWA Primer/Loading Dye Mix | Freezer (~-20°C) | Freezer (~-20°C) | 12 months | 12 months | |
| pBR322/BstNI Markers | Freezer (~-20°C) | Freezer (~-20°C) | 12 months | 12 months | |
| McrBc Buffer, 10× | Freezer (~-20°C) | Freezer (~-20°C) | 3 months | 12 months | |
| McrBc Enzyme, 10 U/µL, 20 µL | Freezer (~-20°C) | Freezer (~-20°C) | Check expiration date on label-usually 1-6 months from receipt | ||
| Guanosine 5′–Triphosphate (GTP), 100 mM, 15 µL | Freezer (~-20°C) | Freezer (~-20°C) | 3 months | 12 months | |
| BSA, 10 mg/mL, 30 µL | Freezer (~-20°C) | Freezer (~-20°C) | 12 months | 12 months | |
| PCLONE RED PLASMID, 100UL | Refrigerator (~4°C) | Freezer (~-20°C) | 2 weeks | 6-12 months | |
| PCLONE BLUE PLASMID, 100UL | Refrigerator (~4°C) | Freezer (~-20°C) | 2 weeks | 6-12 months | |
| E COLI W/PLAC RFP PLASMID | Refrigerator (~4°C) | Refrigerator (~4°C) | 2 weeks | 2 weeks | |
| E COLI W/P5AMILCP BLUE PLASMID | Refrigerator (~4°C) | Refrigerator (~4°C) | 2 weeks | 2 weeks | |
| 10X LIGASE BUFF(PROMEGA),45 UL | Freezer (~-20°C) | Freezer (~-20°C) | 2 weeks | 2 weeks | |
| T4 DNA LIGASE, PROMEGA, 22UL | Freezer (~-20°C) | Freezer (~-20°C) | 2 weeks | 2 weeks | |
| BSAL RESTRICTION ENZYME, 22UL | Freezer (~-20°C) | Freezer (~-20°C) | 2 weeks | 2 weeks | |
| P5 TOP OLIGO, 25 UM, 50UL | Freezer (~-20°C) | Freezer (~-20°C) | 12 months | 12 months | |
| P5 BOT OLIGO, 25 UM, 50UL | Freezer (~-20°C) | Freezer (~-20°C) | 12 months | 12 months | |
| P5-33A TOP OLIGO, 25 UM, 50UL | Freezer (~-20°C) | Freezer (~-20°C) | 12 months | 12 months | |
| P5-33A BOT OLIGO, 25 UM, 50 UL | Freezer (~-20°C) | Freezer (~-20°C) | 12 months | 12 months | |
| AMPICILLIN, 10MG/ML,4ML | Refrigerator (~4°C) | Freezer (~-20°C) | 2 weeks | 6-12 months | |
| MM294 SLANT CULTURE | Refrigerator (~4°C) | Refrigerator (~4°C) | 2 weeks | 2 weeks | |
| DNA SAMPLES | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| MARKERS | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Agarose | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| TBE Buffer | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| GelGreen Stain | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| CarolinaBLU™ Stain | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| DNA SAMPLES | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| MARKERS | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| Agarose | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| TBE Buffer | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| GelGreen Stain | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| CarolinaBLU™ Stain | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| MM294 SLANT CULTURE | Refrigerator (~4°C) | Refrigerator (~4°C) | 2 weeks | 2 weeks | |
| MM294/PAMP SLANT CULTURE | Refrigerator (~4°C) | Refrigerator (~4°C) | 2 weeks | 2 weeks | |
| AMPICILLIN, 10MG/ML,4ML | Refrigerator (~4°C) | Freezer (~-20°C) | 2 weeks | 6-12 months | |
| AMPICILLIN, 100 MG/ML, 200UL | Refrigerator (~4°C) | Freezer (~-20°C) | 2 weeks | 6-12 months | |
| HEMOGLOBIN SAMPLES | Freezer (~-20°C) | Freezer (~-20°C) | 6-12 months | 6-12 months | |
| 5X PROT LOAD DYE NATIGELS300UL | Refrigerator (~4°C) | Refrigerator (~4°C) | 6-12 months | 6-12 months | |
| SODIUM ALGINATE POWDER, 2 G | Room Temp. (~21°C) | Room Temp. (~21°C) | 6 months | 6 months | |
| LACT TABLET,9000 ENZ ACT UNITS | Room Temp. (~21°C) | Room Temp. (~21°C) | 12 months | 12 months | |
| TEST STRIP,URINARY GLUCOSE | Room Temp. (~21°C) | Room Temp. (~21°C) | 6 months | 6 months | |
| YEAST, STRAIN HAO | Refrigerator (~4°C) | Refrigerator (~4°C) | 2 weeks | 2 weeks | |
| YEAST,STRAIN UV SENSITIVE | Refrigerator (~4°C) | Refrigerator (~4°C) | 2 weeks | 2 weeks | |
| MELT AND POUR YED MEDIA 350 ML | Room Temp. (~21°C) | Room Temp. (~21°C) | 6 months | 6 months | |
11/17/23 AL
Care guide
The common fruit fly, Drosophila melanogaster, is the organism most widely used in genetic studies. It is also an excellent live food.
Immediate Care & Handling
When cultures arrive, remove the caps but leave the plugs in place. Keep cultures out of direct sunlight, and maintain at 20° to 25° C (68° to 77° F).
Culturing
It is easy to culture the fruit fly and its generation time is only 2 weeks at 21° C (70° F). The flies develop more slowly if you maintain the culture at a lower temperature, while keeping the culture at a higher temperature may promote male sterility, growth of bacteria and fungi, and mite infestation.
Use transparent vials (item #173120) or glass or plastic bottles (item #173135) as culture vessels for Drosophila. We recommend Carolina’s Formula 4-24® Instant Drosophila Medium (item #173200, #173202, #173204, #173218, #173210, #173212, #173214, or #173216). Follow the directions sent with the medium to set up vials for new cultures. Food medium should be moist at all times, as dry food will inhibit larval growth and result in few flies eclosing. Transfer flies to new culture vials every 10 to 14 days.
For more detailed information on setting up cultures and making crosses, see the Carolina™ Drosophila Manual (item #452620).
FAQs
Which type of Drosophila should I order to feed my mantis (or small frog or reptile)?
We recommend our flightless flies culture (item #144455). Because they cannot fly, they seldom escape from a terrarium or habitat with a fine mesh screen.
What materials do you recommend for culturing Drosophila?
Our Drosophila Culture Kit (item #173050) has everything you need except for the starter cultures. It also comes with the Carolina™ Drosophila Manual, which explains the procedures for culturing and doing Drosophila crosses, and gives information on crosses that are widely used in genetics labs.
Which cultures should I order for a beginning genetics lab?
In the Drosophila cultures section of our print catalog, the list of strains is color coded to indicate those with easy-to-recognize phenotypes that are good for beginning students. Among the most widely used are vestigial crossed with wild type to give a monohybrid cross; vestigial crossed with sepia for a dihybrid cross; and white crossed with wild type for a sex-linked cross. We list F1 cultures of these crosses for your convenience.
Care guide
Elodea densa is an aquatic plant widely used in biology for observation of plant cells and for studies of photosynthesis. This CareSheet covers care of Elodea for lab use. For use of Elodea as an aquarium plant, see our Aquatic Plants Carolina™ CareSheet.
Immediate care and handling
We ship Elodea (items #162101, #162102, and #162103) in a plastic bag without water. (The plant is also available as Elodea Tips [item #157340] in a jar filled with water.) Open the bag and examine the plants. Elodea can vary in color from dark brown to bright green. As long as the plants are not mushy and breaking down, they are healthy. Rinse the plants under running tap water, then shake them gently to remove excess water before use.
Care of the plants before your lab
Although you can use the plants immediately after rinsing them, it is usually best to transfer them to water and put them under lights for 2 days before using them in a lab. This allows the plants time to recover from any shipping shock. Shallow containers with a broad surface area are best. Use either tap water treated to remove chlorine and chloramines (see our “General Guidelines on Living Materials from Carolina Biological Supply Company” on www.carolina.com for details) or spring water. We do not recommend distilled or deionized water, as these lack minerals needed by the plants. Clip the rubber bands from the stems and spread the plants into a single layer in the water. A fluorescent light bank (such as our item #158999) or a 23- to 32-W compact fluorescent bulb in a desk lamp will provide the intense light needed by the plants.
If you must keep the plants for several days before lab use, replace about ¼ of the water every third day. This is to prevent mineral depletion.
Using Elodea to observe plant cells, chloroplasts, and cyclosis
Have students remove, with forceps, a leaf from near the growing tip of an Elodea plant. Place this leaf on a microscope slide in a couple drops of water and add a coverslip. Observe under a microscope at 40×, 100×, and 400×. Students will see cell walls and chloroplasts. They may notice that the chloroplasts of many cells are located along the inner cell wall. This is because a large vacuole occupies the central portion of the cell and tightly presses the cytoplasm against the cell wall.
Students will sometimes notice that the chloroplasts in a cell are moving. This results from movement of the cytoplasm, which is termed cyclosis. As the cytoplasm flows, the chloroplasts move along with it. Light and heat stimulate cyclosis. Tungsten or halogen substage lamps produce both heat and light, and after 2 to 3 minutes students will begin to observe cyclosis. If your microscopes have fluorescent or LED lamps, these produce very little heat and often will not stimulate cyclosis. To provide the needed heat, use a desk lamp equipped with a halogen bulb. Position the lamp so that it shines down on the lab bench. After a few minutes, the surface of the lab bench should become noticeably warm to the touch. Students can place their slides on this warm surface for 3 minutes and then observe for signs of cyclosis. A somewhat better arrangement is to position the lamp so that it shines directly onto the stage of a microscope, thus heating the slide as students view it. Although not all slides may show cyclosis, enough will so that students can share them and see the movement.
Substitutes for Elodea densa
Several states ban Elodea densa. If you live in one of those states, we must ship you a substitute. Here is some information on the plants we commonly send as substitutes.
Elodea canadensis (item #162111): This native plant is similar in appearance to E. densa but is a smaller plant and the leaves typically do not cluster as closely on the stem. Students can observe cells, cell walls, and cyclosis as with E. densa. E. canadensis is a good oxygen producer for studies of photosynthesis and can be shipped to most states. It is often unavailable in the winter months.
Elodea najas: Almost identical in appearance to Elodea densa and can substitute for all uses of that plant. Students can observe cells, cell walls, and cyclosis as with E. densa. E. najas is a good oxygen producer for studies of photosynthesis and can be shipped to all states. There are times when it is unavailable.
Chara (item #162120): The plants (Chara is a multicellular green algae) consist of stems with whorls of branchlets. It is a good oxygen producer for studies of photosynthesis. When viewed under magnification, the internodal cells (stems) show cyclosis; however, this is visualized as streaming of granules within the cytoplasm, not as movement of chloroplasts. The large chloroplast, which forms a background of green, does not move. Chara can be shipped to all states. Chara often has a strong odor, something like garlic. This does not indicate that the plants are dead or decaying.
Disposal
Elodea densa is a non-native plant that is invasive in many areas. After lab use, add leftover plants to an aquarium. Otherwise, seal the plants in a plastic bag, freeze, and discard the unopened bag in the trash.
FAQs
How can I use Elodea to study photosynthesis?
During photosynthesis, the plant absorbs carbon dioxide from the water and releases oxygen. Since oxygen is much less soluble in water than is carbon dioxide, the water quickly saturates with oxygen and bubbles form. The number of bubbles released per minute measures the rate of photosynthesis. Alternately, use a simple volumeter to measure the increase in volume of gas caused by the release of oxygen.
What is the difference between Elodea (item #162101) and Elodea Tips (item #157340)?
Elodea (item #162101) consists of Elodea sprigs that are about 11 cm (4¼”) in length. We recommend these for aquarium plants and for studies of photosynthesis, although they can be used also for cell studies. Elodea Tips (item #157340) are the terminal 3- to 4-cm ends cut from sprigs. We recommend these as best for cell studies.
Can I substitute other plants for Elodea densa?
For photosynthesis studies, almost any aquatic plant will work. These include Ludwigia (item #162141), Cabomba (item #162022), Myriophyllum (item #162161), Ceratophyllum (item #162041), and Sagittaria (item #162201). For easy viewing of plant cells with chloroplasts, consider Fern Prothallia (item #156877).
Problems?
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